Nogueira, F. T., Madi, S., Chitwood, D. H., Juarez, M. T., Timmermans, M. C. P. (April 2007) Two small regulatory RNAs establish opposing fates of a developmental axis. Genes Dev, 21 (7). pp. 750-5. ISSN 0890-9369 (Print)
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Abstract
Small RNAs are important regulators of gene expression. In maize, adaxial/abaxial (dorsoventral) leaf polarity is established by an abaxial gradient of microRNA166 (miR166), which spatially restricts the expression domain of class III homeodomain leucine zipper (HD-ZIPIII) transcription factors that specify adaxial/upper fate. Here, we show that leafbladeless1 encodes a key component in the trans-acting small interfering RNA (ta-siRNA) biogenesis pathway that acts on the adaxial side of developing leaves and demarcates the domains of hd-zipIII and miR166 accumulation. Our findings indicate that tasiR-ARF, a ta-siRNA, and miR166 establish opposing domains along the adaxial-abaxial axis, thus revealing a novel mechanism of pattern formation.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Meristem leaf polarity pattern formation microRNA TRANS-ACTING SIRNAS |
| Subjects: | organism description > plant > maize organism description > plant bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > sRNA bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > siRNA bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor |
| CSHL Authors: | |
| Communities: | CSHL labs > Timmermans lab School of Biological Sciences > Publications |
| Depositing User: | CSHL Librarian |
| Date: | 1 April 2007 |
| Date Deposited: | 07 Nov 2011 19:02 |
| Last Modified: | 22 Sep 2014 15:15 |
| PMCID: | PMC1838527 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/23108 |
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