A MicroRNA feedback circuit in midbrain dopamine neurons

Kim, J., Inoue, K., Ishii, J., Vanti, W. B., Voronov, S. V., Murchison, E., Hannon, G. J., Abeliovich, A. (August 2007) A MicroRNA feedback circuit in midbrain dopamine neurons. Science, 317 (5842). pp. 1220-4. ISSN 1095-9203 (Electronic) 1095-9203 (Linking)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/17761882

DOI: 10.1126/science.1140481

Abstract

MicroRNAs (miRNAs) are evolutionarily conserved, 18- to 25-nucleotide, non-protein coding transcripts that posttranscriptionally regulate gene expression during development. miRNAs also occur in postmitotic cells, such as neurons in the mammalian central nervous system, but their function is less well characterized. We investigated the role of miRNAs in mammalian midbrain dopaminergic neurons (DNs). We identified a miRNA, miR-133b, that is specifically expressed in midbrain DNs and is deficient in midbrain tissue from patients with Parkinson's disease. miR-133b regulates the maturation and function of midbrain DNs within a negative feedback circuit that includes the paired-like homeodomain transcription factor Pitx3. We propose a role for this feedback circuit in the fine-tuning of dopaminergic behaviors such as locomotion.

Item Type:	Paper
Uncontrolled Keywords:	3' Untranslated Regions/metabolism Aged Aged, 80 and over Animals Cell Differentiation Cell Line Cells, Cultured Dopamine/ metabolism Embryonic Stem Cells Feedback, Physiological Female Gene Expression Regulation Homeodomain Proteins/ metabolism Humans Locomotion Male Mesencephalon/cytology/ metabolism Mice MicroRNAs/ metabolism Middle Aged Models, Biological Neurons/cytology/ metabolism Parkinson Disease/metabolism Rats Ribonuclease III/genetics/metabolism Transcription Factors/ metabolism Transcription, Genetic
Subjects:	diseases & disorders > nervous system diseases and disorders > Parkinson's disease organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dopaminergic neuron organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dopaminergic neuron organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dopaminergic neuron organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > dopaminergic neuron organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > dopaminergic neuron organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > dopaminergic neuron bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA organism description > animal > mammal organs, tissues, organelles, cell types and functions > tissues types and functions > midbrain
CSHL Authors:	Hannon, Gregory J. Murchison, Elizabeth P.
Communities:	CSHL labs > Hannon lab School of Biological Sciences > Publications
Depositing User:	CSHL Librarian
Date:	31 August 2007
Date Deposited:	11 Nov 2011 17:48
Last Modified:	22 Sep 2014 15:06
PMCID:	PMC2782470
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/23067

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