Requirement of cytochrome c for apoptosis in human cells

Matapurkar, A., Lazebnik, Y. (December 2006) Requirement of cytochrome c for apoptosis in human cells. Cell Death Differ, 13 (12). pp. 2062-7. ISSN 1350-9047 (Print)

DOI: 10.1038/sj.cdd.4401968


During apoptosis, cytochrome c released from mitochondria activates Apaf-1, a cofactor of caspase-9. The evidence that cytochrome c can activate Apaf-1 is abundant, but the proof that cytochrome c is required for apoptosis is limited to two studies that used genetically modified mice. One of these studies concluded that in some tissues apoptosis may require Apaf-1 but not cytochrome c, which indicated the need to analyze the requirement of cytochrome c beyond the mouse models, and in human tumor cells in particular. In this study, we designed tools to silence cytochrome c expression in human cells and tested these tools in an experimental system of oncogenic transformation. We found that cytochrome c was required for apoptosis induced by both DNA damage and, unexpectedly, TNFalpha. Overall, this study established that cytochrome c is required for apoptosis in human cells and provided tools to dissect mechanisms of apoptosis in various experimental models.

Item Type: Paper
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > apoptosis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene silencing
CSHL Authors:
Communities: CSHL labs > Labeznik lab
Depositing User: CSHL Librarian
Date: December 2006
Date Deposited: 12 Dec 2011 18:56
Last Modified: 19 Apr 2018 18:50
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