Matapurkar, A., Lazebnik, Y. (December 2006) Requirement of cytochrome c for apoptosis in human cells. Cell Death Differ, 13 (12). pp. 2062-7. ISSN 1350-9047 (Print)
Abstract
During apoptosis, cytochrome c released from mitochondria activates Apaf-1, a cofactor of caspase-9. The evidence that cytochrome c can activate Apaf-1 is abundant, but the proof that cytochrome c is required for apoptosis is limited to two studies that used genetically modified mice. One of these studies concluded that in some tissues apoptosis may require Apaf-1 but not cytochrome c, which indicated the need to analyze the requirement of cytochrome c beyond the mouse models, and in human tumor cells in particular. In this study, we designed tools to silence cytochrome c expression in human cells and tested these tools in an experimental system of oncogenic transformation. We found that cytochrome c was required for apoptosis induced by both DNA damage and, unexpectedly, TNFalpha. Overall, this study established that cytochrome c is required for apoptosis in human cells and provided tools to dissect mechanisms of apoptosis in various experimental models.
| Item Type: | Paper |
|---|---|
| Subjects: | organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > apoptosis bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene silencing |
| CSHL Authors: | |
| Communities: | CSHL labs > Labeznik lab |
| Depositing User: | CSHL Librarian |
| Date: | December 2006 |
| Date Deposited: | 12 Dec 2011 18:56 |
| Last Modified: | 19 Apr 2018 18:50 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/22860 |
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