Gurden, H., Uchida, N., Mainen, Z. F. (October 2006) Sensory-evoked intrinsic optical signals in the olfactory bulb are coupled to glutamate release and uptake. Neuron, 52 (2). pp. 335-45. ISSN 0896-6273 (Print)
Abstract
Functional imaging signals arise from metabolic and hemodynamic activity, but how these processes are related to the synaptic and electrical activity of neurons is not well understood. To provide insight into this issue, we used in vivo imaging and simultaneous local pharmacology to study how sensory-evoked neural activity leads to intrinsic optical signals (IOS) in the well-defined circuitry of the olfactory glomerulus. Odor-evoked IOS were tightly coupled to release of glutamate and were strongly modulated by activation of presynaptic dopamine and GABA-B receptors. Surprisingly, IOS were independent of postsynaptic transmission through ionotropic or metabotropic glutamate receptors, but instead were inhibited when uptake by astrocytic glutamate transporters was blocked. These data suggest that presynaptic glutamate release and uptake by astrocytes form a critical pathway through which neural activity is linked to metabolic processing and hence to functional imaging signals.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Animals Astrocytes drug effects metabolism secretion Cell Communication physiology Cerebrovascular Circulation physiology Dopamine metabolism Energy Metabolism physiology Excitatory Amino Acid Transporter metabolism Excitatory Amino Acid Transporter 2 antagonists & inhibitors metabolism Glutamic Acid metabolism Light Models Neurological Olfactory Bulb metabolism Optics Presynaptic Terminals metabolism Rats Rats Long-Evans Receptors, Dopamine/metabolism Receptors GABA-B metabolism Receptors Glutamate metabolism Synaptic Transmission physiology gamma-Aminobutyric Acid metabolism |
| Subjects: | Investigative techniques and equipment > electrophysiology Investigative techniques and equipment > imaging organism description > animal behavior > odor recognition organs, tissues, organelles, cell types and functions > tissues types and functions > olfactory bulb organs, tissues, organelles, cell types and functions > sub-cellular tissues: types and functions > synapse |
| CSHL Authors: | |
| Communities: | CSHL labs > Mainen lab |
| Depositing User: | CSHL Librarian |
| Date: | 19 October 2006 |
| Date Deposited: | 15 Dec 2011 15:12 |
| Last Modified: | 13 Apr 2018 19:53 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/22807 |
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