Andl, T., Murchison, E. P., Liu, Fei, Zhang, Yi, Yunta-Gonzalez, M., Tobias, J. W., Andl, C. D., Seykora, J. T., Hannon, G. J., Millar, S. E.
(May 2006)
The miRNA-Processing Enzyme Dicer Is Essential for the Morphogenesis and Maintenance of Hair Follicles.
Current Biology, 16 (10).
pp. 1041-1049.
ISSN 09609822
Abstract
The discovery that microRNAs (miRNAs) play important roles in regulating gene expression via posttranscriptional repression has revealed a previously unsuspected mechanism controlling development and progenitor-cell function (reviewed in [1, 2]); however, little is known of miRNA functions in mammalian organogenesis. Processing of miRNAs and their assembly into the RNA-induced silencing (RISC) complex requires the essential multifunctional enzyme Dicer [1]. We found that Dicer mRNA and multiple miRNAs are expressed in mouse skin, suggesting roles in skin- and hair-follicle biology. In newborn mice carrying an epidermal-specific Dicer deletion, hair follicles were stunted and hypoproliferative. Hair-shaft and inner-root-sheath differentiation was initiated, but the mutant hair follicles were misoriented and expression of the key signaling molecules Shh and Notch1 was lost by postnatal day 7. At this stage, hair-follicle dermal papillae were observed to evaginate, forming highly unusual structures within the basal epidermis. Normal hair shafts were not produced in the Dicer mutant, and the follicles lacked stem cell markers and degenerated. In contrast to decreased follicular proliferation, the epidermis became hyperproliferative. These results reveal critical roles for Dicer in the skin and implicate miRNAs in key aspects of epidermal and hair-follicle development and function. © 2006 Elsevier Ltd. All rights reserved.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
RNA
DEVBIO
Stemcell |
| Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > dicer
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > dicer
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > endothelial progenitor cell
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > endothelial progenitor cell
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > endothelial progenitor cell
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > progenitor cell > endothelial progenitor cell
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > progenitor cell > endothelial progenitor cell
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > progenitor cell > endothelial progenitor cell
organs, tissues, organelles, cell types and functions > tissues types and functions > epithelium
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene silencing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mRNA dynamics
organism description > animal > mammal > rodent > mouse
organs, tissues, organelles, cell types and functions > organs types and functions > organogenesis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression > post transcriptional modification
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > progenitor cell
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > progenitor cell
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > progenitor cell |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Hannon lab
School of Biological Sciences > Publications |
| Depositing User: |
CSHL Librarian
|
| Date: |
23 May 2006 |
| Date Deposited: |
20 Dec 2011 17:55 |
| Last Modified: |
19 Sep 2014 16:05 |
| PMCID: |
PMC2996092 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/22748 |
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