Navin, N. E., Krasnitz, A., Rodgers, L., Cook, K., Meth, J. L., Kendall, J. T., Riggs, M., Eberling, Y., Troge, J. E., Grubor, V., Levy, D., Lundin, P., Månér, S., Zetterberg, A., Hicks, J. B., Wigler, M. H. (January 2010) Inferring tumor progression from genomic heterogeneity. Genome Research, 20 (1). pp. 68-80.
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Abstract
Cancer progression in humans is difficult to infer because we do not routinely sample patients at multiple stages of their disease. However, heterogeneous breast tumors provide a unique opportunity to study human tumor progression because they still contain evidence of early and intermediate subpopulations in the form of the phylogenetic relationships. We have developed a method we call Sector-Ploidy-Profiling (SPP) to study the clonal composition of breast tumors. SPP involves macro-dissecting tumors, flow-sorting genomic subpopulations by DNA content, and profiling genomes using comparative genomic hybridization (CGH). Breast carcinomas display two classes of genomic structural variation: (1) monogenomic and (2) polygenomic. Monogenomic tumors appear to contain a single major clonal subpopulation with a highly stable chromosome structure. Polygenomic tumors contain multiple clonal tumor subpopulations, which may occupy the same sectors, or separate anatomic locations. In polygenomic tumors, we show that heterogeneity can be ascribed to a few clonal subpopulations, rather than a series of gradual intermediates. By comparing multiple subpopulations from different anatomic locations, we have inferred pathways of cancer progression and the organization of tumor growth. © 2010 by Cold Spring Harbor Laboratory Press.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | tumor progression Sector-Ploidy-Profiling (SPP) comparative genomic HYBRIDIZATION (CGH) |
| Subjects: | bioinformatics > genomics and proteomics diseases & disorders > cancer > cancer types > breast cancer organism description > animal > mammal > primates > hominids > human diseases & disorders > cancer > cancer types |
| CSHL Authors: | |
| Communities: | CSHL Cancer Center Shared Resources > Flow Cytometry Service CSHL Cancer Center Shared Resources > Instrumentation Service CSHL Cancer Center Shared Resources > Microscopy Service CSHL labs > Hicks lab CSHL labs > Krasnitz lab CSHL labs > Levy lab CSHL labs > Wigler lab School of Biological Sciences > Publications |
| Depositing User: | CSHL Librarian |
| Date: | January 2010 |
| Date Deposited: | 05 Oct 2011 15:18 |
| Last Modified: | 07 Jan 2019 16:54 |
| PMCID: | PMC2798832 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/15494 |
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