Probing the initiation and effector phases of the somatic piRNA pathway in Drosophila

Haase, A. D., Fenoglio, S., Muerdter, F., Guzzardo, P. M., Czech, B., Pappin, D. J., Chen, C., Gordon, A., Hannon, G. J. (October 2010) Probing the initiation and effector phases of the somatic piRNA pathway in Drosophila. Genes and Development, 24 (22). pp. 2499-2504. ISSN 08909369 (ISSN)

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Abstract

Combining RNAi in cultured cells and analysis of mutant animals, we probed the roles of known Piwi-interacting RNA (piRNA) pathway components in the initiation and effector phases of transposon silencing. Squash associated physically with Piwi, and reductions in its expression led to modest transposon derepression without effects on piRNAs, consistent with an effector role. Alterations in Zucchini or Armitage reduced both Piwi protein and piRNAs, indicating functions in the formation of a stable Piwi RISC (RNA-induced silencing complex). Notably, loss of Zucchini or mutations within its catalytic domain led to accumulation of unprocessed precursor transcripts from flamenco, consistent with a role for this putative nuclease in piRNA biogenesis.

Item Type:	Paper
Subjects:	organism description > animal > insect > Drosophila bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNAi bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > piRNA
CSHL Authors:	Czech, Benjamin Fenoglio, Silvia Gordon, Assaf Guzzardo, Paloma M. Haase, Astrid D. Hannon, Gregory J. Muerdter, Felix Pappin, Darryl J.
Communities:	CSHL Post Doctoral Fellows CSHL labs > Hannon lab CSHL labs > Pappin lab School of Biological Sciences > Publications CSHL Cancer Center Shared Resources > Bioinformatics Service CSHL Cancer Center Shared Resources > DNA Sequencing Service CSHL Cancer Center Shared Resources > Microscopy Service
Depositing User:	CSHL Librarian
Date:	21 October 2010
Date Deposited:	30 Sep 2011 13:21
Last Modified:	26 Dec 2014 20:52
PMCID:	PMC2975926
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/15426

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