DeBlasio, S. L., Sylvester, A. W., Jackson, D. P. (March 2010) Illuminating plant biology: using fluorescent proteins for high-throughput analysis of protein localization and function in plants. Briefings in Functional Genomics, 9 (2). pp. 129-138. ISSN 2041-2649
Abstract
First discovered in jellyfish, fluorescent proteins (FPs) have been successfully optimized for use as effective biomarkers within living plant cells. When exposed to light, FPs fused to a protein or regulatory element will fluoresce, and non-invasively mark expression and protein localization, which allows for the in vivo monitoring of diverse cellular processes. In this review, we discuss how FP technology has evolved from small-scale analysis of individual genes to more high-throughput techniques for global expression and functional profiling in plants.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | fluorescent proteins maize Arabidopsis high-throughput techniques global expression functional profiling TARGETED GENE-EXPRESSION ENHANCER TRAP LINES CELL-TO-CELL ARABIDOPSIS-THALIANA FUSION PROTEIN DEVELOPMENTAL PLASTICITY TRANSGENE EXPRESSION EPIDERMAL-CELLS APICAL MERISTEM ROOT-MERISTEM |
| Subjects: | organism description > plant > Arabidopsis organism description > plant > maize bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > green fluorescent protein |
| CSHL Authors: | |
| Communities: | CSHL labs > Jackson lab |
| Depositing User: | CSHL Librarian |
| Date: | March 2010 |
| Date Deposited: | 29 Sep 2011 20:24 |
| Last Modified: | 08 Apr 2015 13:46 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/15397 |
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