The transcription factor TCFL5 responds to A MYB to elaborate the male meiotic program in mice

Cecchini, Katharine, Biasini, Adriano, Yu, Tianxiong, Säflund, Martin, Mou, Haiwei, Arif, Amena, Eghbali, Atiyeh, Colpan, Cansu, Gainetdinov, Ildar, de Rooij, Dirk G, Weng, Zhiping, Zamore, Phillip, Ozata, Deniz M (November 2022) The transcription factor TCFL5 responds to A MYB to elaborate the male meiotic program in mice. Reproduction. REP-22. ISSN 1470-1626

Abstract

In male mice, the transcription factors STRA8 and MEISON initiate meiosis I. We report that STRA8/MEISON activates the transcription factors A MYB and TCFL5, which together reprogram gene expression after spermatogonia enter into meiosis. TCFL5 promotes transcription of genes required for meiosis, mRNA turnover, miR-34/449 production, meiotic exit, and spermiogenesis. This transcriptional architecture is conserved in rhesus macaque, suggesting TCFL5 plays a central role in meiosis and spermiogenesis in placental mammals. Tcfl5em1/em1 mutants are sterile, and spermatogenesis arrests at the mid- or late-pachytene stage of meiosis. Moreover, Tcfl5+/em1 mutants produce fewer motile sperm.

Item Type:	Paper
Subjects:	bioinformatics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification diseases & disorders bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function organs, tissues, organelles, cell types and functions > organelles, types and functions > meiosis organs, tissues, organelles, cell types and functions > organelles, types and functions diseases & disorders > pregnancy bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor
CSHL Authors:	Mou, Haiwei
Communities:	CSHL labs > Beyaz lab
SWORD Depositor:	CSHL Elements
Depositing User:	CSHL Elements
Date:	1 November 2022
Date Deposited:	12 Dec 2022 14:53
Last Modified:	11 Jan 2024 19:11
PMCID:	PMC9812935
URI:	https://repository.cshl.edu/id/eprint/40768

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