Prostate tumor-induced stromal reprogramming generates Tenascin C that promotes prostate cancer metastasis through YAP/TAZ inhibition

Lee, Yu-Chen, Lin, Song-Chang, Yu, Guoyu, Zhu, Ming, Song, Jian H, Rivera, Keith, Pappin, Darryl J, Logothetis, Christopher J, Panaretakis, Theocharis, Wang, Guocan, Yu-Lee, Li-Yuan, Lin, Sue-Hwa (November 2021) Prostate tumor-induced stromal reprogramming generates Tenascin C that promotes prostate cancer metastasis through YAP/TAZ inhibition. Oncogene. ISSN 0950-9232

URL: https://www.ncbi.nlm.nih.gov/pubmed/34845375
DOI: 10.1038/s41388-021-02131-7

Abstract

Metastatic prostate cancer (PCa) in bone induces bone-forming lesions that enhance PCa progression. How tumor-induced bone formation enhances PCa progression is not known. We have previously shown that PCa-induced bone originates from endothelial cells (ECs) that have undergone endothelial-to-osteoblast (EC-to-OSB) transition by tumor-secreted bone morphogenetic protein 4 (BMP4). Here, we show that EC-to-OSB transition leads to changes in the tumor microenvironment that increases the metastatic potential of PCa cells. We found that conditioned medium (CM) from EC-OSB hybrid cells increases the migration, invasion, and survival of PC3-mm2 and C4-2B4 PCa cells. Quantitative mass spectrometry (Isobaric Tags for Relative and Absolute Quantitation) identified Tenascin C (TNC) as one of the major proteins secreted from EC-OSB hybrid cells. TNC expression in tumor-induced OSBs was confirmed by immunohistochemistry of MDA PCa-118b xenograft and human bone metastasis specimens. Mechanistically, BMP4 increases TNC expression in EC-OSB cells through the Smad1-Notch/Hey1 pathway. How TNC promotes PCa metastasis was next interrogated by in vitro and in vivo studies. In vitro studies showed that a TNC-neutralizing antibody inhibits EC-OSB-CM-mediated PCa cell migration and survival. TNC knockdown decreased, while the addition of recombinant TNC or TNC overexpression increased migration and anchorage-independent growth of PC3 or C4-2b cells. When injected orthotopically, PC3-mm2-shTNC clones decreased metastasis to bone, while C4-2b-TNC-overexpressing cells increased metastasis to lymph nodes. TNC enhances PCa cell migration through α5β1 integrin-mediated YAP/TAZ inhibition. These studies elucidate that tumor-induced stromal reprogramming generates TNC that enhances PCa metastasis and suggest that TNC may be a target for PCa therapy.

Item Type: Paper
Subjects: diseases & disorders > cancer > metastasis
diseases & disorders > cancer > cancer types > prostate cancer
CSHL Authors:
Communities: CSHL labs > Pappin lab
SWORD Depositor: CSHL Elements
Depositing User: CSHL Elements
Date: 29 November 2021
Date Deposited: 06 Dec 2021 19:24
Last Modified: 07 Feb 2022 19:36
PMCID: PMC8818031
URI: https://repository.cshl.edu/id/eprint/40438

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