Standardized and reproducible measurement of decision-making in mice.

International Brain Laboratory, Aguillon-Rodriguez, Valeria, Angelaki, Dora, Bayer, Hannah, Bonacchi, Niccolo, Carandini, Matteo, Cazettes, Fanny, Chapuis, Gaelle, Churchland, Anne K, Dan, Yang, Dewitt, Eric, Faulkner, Mayo, Forrest, Hamish, Haetzel, Laura, Häusser, Michael, Hofer, Sonja B, Hu, Fei, Khanal, Anup, Krasniak, Christopher, Laranjeira, Ines, Mainen, Zachary F, Meijer, Guido, Miska, Nathaniel J, Mrsic-Flogel, Thomas D, Murakami, Masayoshi, Noel, Jean-Paul, Pan-Vazquez, Alejandro, Rossant, Cyrille, Sanders, Joshua, Socha, Karolina, Terry, Rebecca, Urai, Anne E, Vergara, Hernando, Wells, Miles, Wilson, Christian J, Witten, Ilana B, Wool, Lauren E, Zador, Anthony M (May 2021) Standardized and reproducible measurement of decision-making in mice. eLife, 10. ISSN 2050-084X

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Abstract

Progress in science requires standardized assays whose results can be readily shared, compared, and reproduced across laboratories. Reproducibility, however, has been a concern in neuroscience, particularly for measurements of mouse behavior. Here, we show that a standardized task to probe decision-making in mice produces reproducible results across multiple laboratories. We adopted a task for head-fixed mice that assays perceptual and value-based decision making, and we standardized training protocol and experimental hardware, software, and procedures. We trained 140 mice across seven laboratories in three countries, and we collected 5 million mouse choices into a publicly available database. Learning speed was variable across mice and laboratories, but once training was complete there were no significant differences in behavior across laboratories. Mice in different laboratories adopted similar reliance on visual stimuli, on past successes and failures, and on estimates of stimulus prior probability to guide their choices. These results reveal that a complex mouse behavior can be reproduced across multiple laboratories. They establish a standard for reproducible rodent behavior, and provide an unprecedented dataset and open-access tools to study decision-making in mice. More generally, they indicate a path toward achieving reproducibility in neuroscience through collaborative open-science approaches.

Item Type:	Paper
Subjects:	organism description > animal organism description > animal behavior organism description > animal behavior > decision making organism description > animal behavior > learning organism description > animal > mammal organism description > animal > mammal > rodent > mouse neurobiology neurobiology > neuroscience organism description > animal behavior > perception organism description > animal > mammal > rodent
CSHL Authors:	Churchland, Anne K. Khanal, Anup Urai, Anne Zador, Anthony M. Krasniak, Christopher
Communities:	CSHL labs > Churchland lab CSHL labs > Zador lab School of Biological Sciences > Publications
SWORD Depositor:	CSHL Elements
Depositing User:	CSHL Elements
Date:	20 May 2021
Date Deposited:	26 May 2021 15:36
Last Modified:	24 Jan 2024 21:17
PMCID:	PMC8137147
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/40172

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