Deciphering the regulatory genome of Escherichia coli, one hundred promoters at a time.

Ireland, William T, Beeler, Suzannah M, Flores-Bautista, Emanuel, McCarty, Nicholas S, Röschinger, Tom, Belliveau, Nathan M, Sweredoski, Michael J, Moradian, Annie, Kinney, Justin B, Phillips, Rob (September 2020) Deciphering the regulatory genome of Escherichia coli, one hundred promoters at a time. eLife, 9. pp. 1-76. ISSN 2050-084X

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URL: https://www.ncbi.nlm.nih.gov/pubmed/32955440
DOI: 10.7554/eLife.55308

Abstract

Advances in DNA sequencing have revolutionized our ability to read genomes. However, even in the most well-studied of organisms, the bacterium Escherichia coli, for ≈65% of promoters we remain ignorant of their regulation. Until we crack this regulatory Rosetta Stone, efforts to read and write genomes will remain haphazard. We introduce a new method, Reg-Seq, that links massively parallel reporter assays with mass spectrometry to produce a base pair resolution dissection of more than a E. coli promoters in 12 growth conditions. We demonstrate that the method recapitulates known regulatory information. Then, we examine regulatory architectures for more than 80 promoters which previously had no known regulatory information. In many cases, we also identify which transcription factors mediate their regulation. This method clears a path for highly multiplexed investigations of the regulatory genome of model organisms, with the potential of moving to an array of microbes of ecological and medical relevance.

Item Type: Paper
Subjects: organism description > bacteria
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > genomes
CSHL Authors:
Communities: CSHL labs > Kinney lab
SWORD Depositor: CSHL Elements
Depositing User: CSHL Elements
Date: 21 September 2020
Date Deposited: 07 May 2021 14:12
Last Modified: 07 May 2021 14:12
PMCID: PMC7567609
Related URLs:
URI: https://repository.cshl.edu/id/eprint/40045

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