Diverse Macrophage Populations Contribute to the Inflammatory Microenvironment in Premalignant Lesions During Localized Invasion.

Ibrahim, Ayman M, Moss, Matthew A, Gray, Zane, Rojo, Michelle D, Burke, Caitlin M, Schwertfeger, Kathryn L, Dos Santos, Camila O, Machado, Heather L (2020) Diverse Macrophage Populations Contribute to the Inflammatory Microenvironment in Premalignant Lesions During Localized Invasion. Frontiers in Oncology, 10. p. 569985. ISSN 2234-943X

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Abstract

Myeloid cell heterogeneity remains poorly studied in breast cancer, and particularly in premalignancy. Here, we used single cell RNA sequencing to characterize macrophage diversity in mouse pre-invasive lesions as compared to lesions undergoing localized invasion. Several subpopulations of macrophages with transcriptionally distinct profiles were identified, two of which resembled macrophages in the steady state. While all subpopulations expressed tumor-promoting genes, many of the populations expressed pro-inflammatory genes, differing from reports in tumor-associated macrophages. Gene profiles of the myeloid cells were similar between early and late stages of premalignancy, although expansion of some subpopulations occurred. These results unravel macrophage heterogeneity in early progression and may provide insight into early intervention strategies that target macrophages.

Item Type:	Paper
Subjects:	diseases & disorders > cancer > cancer types > breast cancer organs, tissues, organelles, cell types and functions > cell types and functions > cell types > macrophages organs, tissues, organelles, cell types and functions > cell types and functions > cell types > macrophages organs, tissues, organelles, cell types and functions > cell types and functions > cell types > macrophages Investigative techniques and equipment > assays > Single cell sequencing diseases & disorders > cancer > drugs and therapies > tumor microenvironment
CSHL Authors:	Dos Santos, Camila O.
Communities:	CSHL labs > Dos Santos lab
SWORD Depositor:	CSHL Elements
Depositing User:	CSHL Elements
Date:	2020
Date Deposited:	04 May 2021 13:32
Last Modified:	04 May 2021 13:32
PMCID:	PMC7541939
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/39976

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