Identification of the First Nuclear Male Sterility Gene (Male-sterile 9) in Sorghum

Chen, J. P., Jiao, Y. P., Laza, H., Payton, P., Ware, D., Xin, Z. G. (November 2019) Identification of the First Nuclear Male Sterility Gene (Male-sterile 9) in Sorghum. Plant Genome, 12 (3). Article No 190020. ISSN 1940-3372

DOI: 10.3835/plantgenome2019.03.0020


Nuclear male sterility (NMS) is important for understanding microspore development and could facilitate the development of new strategies to control male sterility. Several NMS lines and mutants have been reported in sorghum [Sorghum bicolor (L.) Moench] previously. However, no male-sterile gene has been identified, hampering the utility of NMS in sorghum breeding. In this study, we characterized a new NMS mutant, male sterile 9 (ms9), which is distinct from all other reported NMS loci. The ms9 mutant is stable under a variety of environmental conditions. Homozygous ms9 plants produced normal ovaries but small pale-colored anthers that contained no pollen grains. Microscopic analyses revealed abnormal microspore development of ms9 at the midmicrospore stage, causing degeneration of microspore inside the anther lobes and male sterility of ms9 plants. Using MutMap, we identified the Ms9 gene as a plant homeotic domain (PHD)-finger transcription factor similar to Ms1 in Arabidopsis thaliana (L.) Heynh. and Ptc1 in rice (Oryza sativa L.). Ms9 is the first NMS gene identified in sorghum. Thus, the Ms9 gene and ms9 mutant provide new genetic tools for studying pollen development and controlling male sterility in sorghum.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
organism description > plant
CSHL Authors:
Communities: CSHL labs > Ware lab
Depositing User: Adrian Gomez
Date: November 2019
Date Deposited: 19 Dec 2019 19:29
Last Modified: 29 Jun 2021 19:01
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