lncRNA Spehd Regulates Hematopoietic Stem and Progenitor Cells and Is Required for Multilineage Differentiation

Delas, M. J., Jackson, B. T., Kovacevic, T., Vangelisti, S., Munera Maravilla, E., Wild, S. A., Stork, E. M., Erard, N., Knott, S. R. V., Hannon, G. J. (April 2019) lncRNA Spehd Regulates Hematopoietic Stem and Progenitor Cells and Is Required for Multilineage Differentiation. Cell Rep, 27 (3). 719-729.e6.

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URL: https://www.ncbi.nlm.nih.gov/pubmed/30995471
DOI: 10.1016/j.celrep.2019.03.080

Abstract

Long non-coding RNAs (lncRNAs) show patterns of tissue- and cell type-specific expression that are very similar to those of protein coding genes and consequently have the potential to control stem and progenitor cell fate decisions along a differentiation trajectory. To understand the roles that lncRNAs may play in hematopoiesis, we selected a subset of mouse lncRNAs with potentially relevant expression patterns and refined our candidate list using evidence of conserved expression in human blood lineages. For each candidate, we assessed its possible role in hematopoietic differentiation in vivo using competitive transplantation. Our studies identified two lncRNAs that were required for hematopoiesis. One of these, Spehd, showed defective multilineage differentiation, and its silencing yielded common myeloid progenitors that are deficient in their oxidative phosphorylation pathway. This effort not only suggests that lncRNAs can contribute to differentiation decisions during hematopoiesis but also provides a path toward the identification of functional lncRNAs in other differentiation hierarchies.

Item Type: Paper
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > differentiation
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > hematopoietic cell
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > hematopoietic cell
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > hematopoietic cell
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > long non-coding RNA
CSHL Authors:
Communities: CSHL labs > Hannon lab
Depositing User: Matthew Dunn
Date: 16 April 2019
Date Deposited: 23 Apr 2019 15:40
Last Modified: 23 Apr 2019 15:40
Related URLs:
URI: https://repository.cshl.edu/id/eprint/37786

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