A high-throughput neurohistological pipeline for brain-wide mesoscale connectivity mapping of the common marmoset

Lin, M. K., Takahashi, Y. S., Huo, B. X., Hanada, M., Nagashima, J., Hata, J., Tolpygo, A. S., Ram, K., Lee, B. C., Miller, M. I., Rosa, M. G., Sasaki, E., Iriki, A., Okano, H., Mitra, P. (February 2019) A high-throughput neurohistological pipeline for brain-wide mesoscale connectivity mapping of the common marmoset. Elife, 8. ISSN 2050-084x

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URL: https://www.ncbi.nlm.nih.gov/pubmed/30720427
DOI: 10.7554/eLife.40042


Understanding the connectivity architecture of entire vertebrate brains is a fundamental but difficult task. Here we present an integrated neuro-histological pipeline as well as a grid-based tracer injection strategy for systematic mesoscale connectivity mapping in the common Marmoset (Callithrix jacchus). Individual brains are sectioned into ~1700 20microm sections using the tape transfer technique, permitting high quality 3D reconstruction of a series of histochemical stains (Nissl, myelin) interleaved with tracer labelled sections. Systematic in-vivo MRI of the individual animals facilitates injection placement into reference-atlas defined anatomical compartments. Further, combining the resulting 3D volumes, containing informative cytoarchitectonic markers, with in-vivo and ex-vivo MRI, and using an integrated computational pipeline, we are able to accurately map individual brains into a common reference atlas despite the significant individual variation. This approach will facilitate the systematic assembly of a mesoscale connectivity matrix together with unprecedented 3D reconstructions of brain-wide projection patterns in a primate brain.

Item Type: Paper
Subjects: organs, tissues, organelles, cell types and functions > organs types and functions > brain
organism description > animal > mammal > primates
Investigative techniques and equipment > Whole Brain Circuit Mapping
CSHL Authors:
Communities: CSHL labs > Mitra lab
Depositing User: Matthew Dunn
Date: 5 February 2019
Date Deposited: 08 Feb 2019 21:08
Last Modified: 29 Jun 2021 19:52
PMCID: PMC6384052
Related URLs:
URI: https://repository.cshl.edu/id/eprint/37691

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