Lin, M. K., Takahashi, Y. S., Huo, B. X., Hanada, M., Nagashima, J., Hata, J., Tolpygo, A. S., Ram, K., Lee, B. C., Miller, M. I., Rosa, M. G., Sasaki, E., Iriki, A., Okano, H., Mitra, P. (February 2019) A high-throughput neurohistological pipeline for brain-wide mesoscale connectivity mapping of the common marmoset. Elife, 8. ISSN 2050-084x
Preview |
PDF
2019.Lin.MarmosetBrain.pdf - Published Version Available under License Creative Commons Attribution. Download (20MB) | Preview |
Abstract
Understanding the connectivity architecture of entire vertebrate brains is a fundamental but difficult task. Here we present an integrated neuro-histological pipeline as well as a grid-based tracer injection strategy for systematic mesoscale connectivity mapping in the common Marmoset (Callithrix jacchus). Individual brains are sectioned into ~1700 20microm sections using the tape transfer technique, permitting high quality 3D reconstruction of a series of histochemical stains (Nissl, myelin) interleaved with tracer labelled sections. Systematic in-vivo MRI of the individual animals facilitates injection placement into reference-atlas defined anatomical compartments. Further, combining the resulting 3D volumes, containing informative cytoarchitectonic markers, with in-vivo and ex-vivo MRI, and using an integrated computational pipeline, we are able to accurately map individual brains into a common reference atlas despite the significant individual variation. This approach will facilitate the systematic assembly of a mesoscale connectivity matrix together with unprecedented 3D reconstructions of brain-wide projection patterns in a primate brain.
| Item Type: | Paper |
|---|---|
| Subjects: | Investigative techniques and equipment organism description > animal organs, tissues, organelles, cell types and functions > organs types and functions > brain Investigative techniques and equipment > spectroscopy > magnetic resonance spectroscopy organism description > animal > mammal organism description > animal > mammal > primates > monkey > marmoset organism description > animal > mammal > primates > monkey organs, tissues, organelles, cell types and functions > organs types and functions organs, tissues, organelles, cell types and functions organism description > animal > mammal > primates Investigative techniques and equipment > spectroscopy Investigative techniques and equipment > Whole Brain Circuit Mapping |
| CSHL Authors: | |
| Communities: | CSHL labs > Mitra lab |
| Depositing User: | Matthew Dunn |
| Date: | 5 February 2019 |
| Date Deposited: | 08 Feb 2019 21:08 |
| Last Modified: | 02 Feb 2024 17:02 |
| PMCID: | PMC6384052 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/37691 |
Actions (login required)
 |
Administrator's edit/view item |