In Situ Localization of Small RNAs in Plants

Marco, C. F., Skopelitis, D. S., Timmermans, M. C. P. (January 2019) In Situ Localization of Small RNAs in Plants. Methods Mol Biol, 1932. pp. 159-173. ISSN 1064-3745

DOI: 10.1007/978-1-4939-9042-9_12


Small RNAs have vital roles in numerous aspects of plant biology. Deciphering their precise contributions requires knowledge of a small RNA's spatiotemporal pattern of accumulation. The in situ hybridization protocol described here takes advantage of locked nucleic acid (LNA) oligonucleotide probes to visualize small RNA expression at the cellular level with high sensitivity and specificity. The procedure is optimized for paraffin-embedded plant tissue sections, is applicable to a wide range of plants and tissues, and can be completed within 2-6 days.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA expression
organism description > plant
CSHL Authors:
Communities: CSHL labs > Timmermans lab
Depositing User: Matthew Dunn
Date: January 2019
Date Deposited: 04 Feb 2019 17:51
Last Modified: 04 Feb 2019 17:51
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