Marco, C. F., Skopelitis, D. S., Timmermans, M. C. P. (January 2019) In Situ Localization of Small RNAs in Plants. Methods Mol Biol, 1932. pp. 159-173. ISSN 1064-3745
Abstract
Small RNAs have vital roles in numerous aspects of plant biology. Deciphering their precise contributions requires knowledge of a small RNA's spatiotemporal pattern of accumulation. The in situ hybridization protocol described here takes advantage of locked nucleic acid (LNA) oligonucleotide probes to visualize small RNA expression at the cellular level with high sensitivity and specificity. The procedure is optimized for paraffin-embedded plant tissue sections, is applicable to a wide range of plants and tissues, and can be completed within 2-6 days.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA expression bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA expression bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > oligonucleotide organism description > plant |
| CSHL Authors: | |
| Communities: | CSHL labs > Timmermans lab |
| Depositing User: | Matthew Dunn |
| Date: | January 2019 |
| Date Deposited: | 04 Feb 2019 17:51 |
| Last Modified: | 02 Feb 2024 19:20 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/37679 |
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