Olfactory marker protein (OMP) regulates formation and refinement of the olfactory glomerular map

Albeanu, D. F., Provost, A. C., Agarwal, P., Soucy, E. R., Zak, J. D., Murthy, V. N. (November 2018) Olfactory marker protein (OMP) regulates formation and refinement of the olfactory glomerular map. Nat Commun, 9 (1). p. 5073. ISSN 2041-1723

[thumbnail of 2018.Albeanu.Olfactory.pdf]
Preview
PDF
2018.Albeanu.Olfactory.pdf - Published Version
Available under License Creative Commons Attribution.

Download (6MB) | Preview
URL: https://www.ncbi.nlm.nih.gov/pubmed/30498219
DOI: 10.1038/s41467-018-07544-9

Abstract

Inputs from olfactory sensory neuron (OSN) axons expressing the same type of odorant receptor (OR) converge in the glomerulus of the main olfactory bulb. A key marker of mature OSNs is olfactory marker protein (OMP), whose deletion has been associated with deficits in OSN signal transduction and odor discrimination. Here, we investigate glomerular odor responses and anatomical architecture in mice in which one or both alleles of OMP are replaced by the fluorescent synaptic activity reporter, synaptopHluorin. Functionally heterogeneous glomeruli, that is, ones with microdomains with distinct odor responses, are rare in OMP(+/-) mice, but occur frequently in OMP(-/-) mice. Genetic targeting of single ORs reveals that these microdomains arise from co-innervation of individual glomeruli by OSNs expressing different ORs. This glomerular mistargeting is locally restricted to a few glomerular diameters. Our studies document functional heterogeneity in sensory input within individual glomeruli and uncover its anatomical correlate, revealing an unexpected role for OMP in the formation and refinement of the glomerular map.

Item Type: Paper
Subjects: bioinformatics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > alleles
organism description > animal
organism description > animal behavior
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
organs, tissues, organelles, cell types and functions > tissues types and functions > glomerulus
organism description > animal > mammal
organism description > animal > mammal > rodent > mouse
organism description > animal behavior > olfactory
organs, tissues, organelles, cell types and functions > tissues types and functions > olfactory bulb
organism description > animal > mammal > rodent
CSHL Authors:
Communities: CSHL labs > Albeanu lab
Depositing User: Matthew Dunn
Date: 29 November 2018
Date Deposited: 20 Dec 2018 21:09
Last Modified: 06 Feb 2024 20:42
PMCID: PMC6265328
Related URLs:
URI: https://repository.cshl.edu/id/eprint/37504

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving