Individual components of paired typical NLR immune receptors are regulated by distinct E3 ligases

Dong, O. X., Ao, K., Xu, F., Johnson, K. C. M., Wu, Y., Li, L., Xia, S., Liu, Y., Huang, Y., Rodriguez, E., Chen, X., Chen, S., Zhang, Y., Petersen, M., Li, X. (August 2018) Individual components of paired typical NLR immune receptors are regulated by distinct E3 ligases. Nat Plants, 4 (9). pp. 699-710. ISSN 2055-0278

URL: https://www.ncbi.nlm.nih.gov/pubmed/30082764
DOI: 10.1038/s41477-018-0216-8

Abstract

In plants and animals, nucleotide-binding leucine-rich repeat (NLR) proteins serve as intracellular immune receptors. Defence signalling by NLRs often requires the formation of NLR heteropairs. Our knowledge of the molecular mechanism regulating this process is limited. In a reverse genetic screen to identify the partner of the Arabidopsis typical NLR, SUPRESSOR OF NPR1, CONSTITUTIVE 1 (SNC1), we discovered three NLRs that are redundantly required for SNC1-mediated defence, which were named SIDEKICK SNC1 1 (SIKIC1), SIKIC2 and SIKIC3. Immunoprecipitation-mass spectrometry analyses revealed that SIKIC2 physically associates with SNC1. We also uncovered that the protein level of SIKIC2 is under the control of two previously uncharacterized redundant E3 ubiquitin ligases MUSE1 and MUSE2. As SNC1 accumulation has previously been shown to be regulated by the E3 ubiquitin ligase SCF(CPR1), this report provides evidence that the homeostasis of individual components of partnered typical NLRs is subjected to differential regulation via ubiquitin-mediated protein degradation.

Item Type: Paper
Subjects: organism description > plant > Arabidopsis
bioinformatics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > immunity
organism description > plant
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > ubiquitin ligase
CSHL Authors:
Depositing User: Matthew Dunn
Date: 6 August 2018
Date Deposited: 15 Aug 2018 15:29
Last Modified: 20 Feb 2024 18:39
Related URLs:
URI: https://repository.cshl.edu/id/eprint/37118

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