Kim, S. H., Kwon, C. T., Song, G., Koh, H. J., An, G., Paek, N. C. (January 2018) The rice zebra3 (z3) mutation disrupts citrate distribution and produces transverse dark-green/green variegation in mature leaves. Rice (N Y), 11 (1). p. 1. ISSN 1939-8425 (Print)1939-8425
Abstract
BACKGROUND: Rice zebra mutants are leaf variegation mutants that exhibit transverse sectors of green/yellow or green/white in developing or mature leaves. In most cases, leaf variegation is caused by defects in chloroplast biogenesis pathways, leading to an accumulation of reactive oxygen species in a transverse pattern in the leaves. Here, we examine a new type of leaf variegation mutant in rice, zebra3 (z3), which exhibits transverse dark-green/green sectors in mature leaves and lacks the typical yellow or white sectors. RESULTS: Map-based cloning revealed that the Z3 locus encodes a putative citrate transporter that belongs to the citrate-metal hydrogen symport (CitMHS) family. CitMHS family members have been extensively studied in bacteria and function as secondary transporters that can transport metal-citrate complexes, but whether CitMHS family transporters exist in eukaryotes remains unknown. To investigate whether Z3 acts as a citrate transporter in rice, we measured citrate levels in wild-type leaves and in the dark-green and green sectors of the leaves of z3 mutants. The results showed that citrates accumulated to high levels in the dark-green sectors of z3 mutant leaves, but not in the green sectors as compared with the wild-type leaves. CONCLUSIONS: These results suggest that leaf variegation in the z3 mutant is caused by an unbalanced accumulation of citrate in a transverse pattern in the leaves. Taking these results together, we propose that Z3 plays an important role in citrate transport and distribution during leaf development and is a possible candidate for a CitMHS family member in plants.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | CitMHS family Citrate transporter Leaf variegation Rice Zebra3 (z3) |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function organism description > plant > rice |
| CSHL Authors: | |
| Communities: | CSHL labs > Lippman lab |
| Depositing User: | Matt Covey |
| Date: | 5 January 2018 |
| Date Deposited: | 10 Jan 2018 20:42 |
| Last Modified: | 25 Jan 2018 20:38 |
| PMCID: | PMC5756232 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/35800 |
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