Natural variation and dosage of the HEI10 meiotic E3 ligase control Arabidopsis crossover recombination

Ziolkowski, P. A., Underwood, C. J., Lambing, C., Martinez-Garcia, M., Lawrence, E. J., Ziolkowska, L., Griffin, C., Choi, K., Franklin, F. C., Martienssen, R. A., Henderson, I. R. (February 2017) Natural variation and dosage of the HEI10 meiotic E3 ligase control Arabidopsis crossover recombination. Genes Dev, 31 (3). pp. 306-317. ISSN 0890-9369

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DOI: 10.1101/gad.295501.116


During meiosis, homologous chromosomes undergo crossover recombination, which creates genetic diversity and balances homolog segregation. Despite these critical functions, crossover frequency varies extensively within and between species. Although natural crossover recombination modifier loci have been detected in plants, causal genes have remained elusive. Using natural Arabidopsis thaliana accessions, we identified two major recombination quantitative trait loci (rQTLs) that explain 56.9% of crossover variation in ColxLer F2 populations. We mapped rQTL1 to semidominant polymorphisms in HEI10, which encodes a conserved ubiquitin E3 ligase that regulates crossovers. Null hei10 mutants are haploinsufficient, and, using genome-wide mapping and immunocytology, we show that transformation of additional HEI10 copies is sufficient to more than double euchromatic crossovers. However, heterochromatic centromeres remained recombination-suppressed. The strongest HEI10-mediated crossover increases occur in subtelomeric euchromatin, which is reminiscent of sex differences in Arabidopsis recombination. Our work reveals that HEI10 naturally limits Arabidopsis crossovers and has the potential to influence the response to selection.

Item Type: Paper
Uncontrolled Keywords: Arabidopsis Hei10 Zmm meiosis modifier recombination
Subjects: organism description > plant > Arabidopsis
organs, tissues, organelles, cell types and functions > organelles, types and functions > meiosis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > recombination
CSHL Authors:
Communities: CSHL labs > Martienssen lab
Depositing User: Matt Covey
Date: 1 February 2017
Date Deposited: 02 Mar 2017 17:37
Last Modified: 07 Jul 2021 19:10
PMCID: PMC5358726
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