Immunocytochemical localization of the neuron-specific form of the c-src gene product, pp60c-src(+), in rat brain

Sugrue, M. M., Brugge, J. S., Marshak, D. R., Greengard, P., Gustafson, E. L. (August 1990) Immunocytochemical localization of the neuron-specific form of the c-src gene product, pp60c-src(+), in rat brain. J Neurosci, 10 (8). pp. 2513-27. ISSN 0270-6474 (Print)0270-6474 (Linking)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/1696980

Abstract

Neurons express high levels of a variant form of the c-src gene product, denoted pp60c-src(+), which contains a 6 amino acid insert in the amino-terminal half of the c-src protein. We have determined the localization of pp60c-src(+) in neurons using an affinity-purified anti-peptide antibody, referred to as affi-SB12, that exclusively recognizes this neuron-specific form of the c-src gene product. Using affi-SB12, we examined the distribution of pp60c-src(+) by immunoperoxidase staining of sections through adult rat brains, pp60c-src(+) was widely distributed in rat brain and appeared to be differentially expressed in subpopulations of neurons. The majority of immunoreactive neurons was found in the mesencephalon, cerebellum, pons, and medulla. Telencephalic structures that contained substantial populations of pp60c-src(+)-immunoreactive neurons included layer V of the cerebral cortex and the ventral pallidum. Within individual neurons, pp60c-src(+) immunoreactivity was localized to the cell soma and dendritic processes, while labeling of axons and nerve terminals (puncta) was not as readily detected. Dense accumulations of immunoreactive axons were rare, being most prominent in portions of the inferior and superior olive, and in the spinal trigeminal nucleus. While the regional distribution of pp60c-src(+) immunoreactivity does not correlate with any specific neuronal cell type or first messenger system, this unique pattern of expression of pp60c-src(+) suggests the existence of a previously uncharacterized functional organization within the brain. Furthermore, the localization of this neuron-specific tyrosine kinase in functionally important areas of the nerve cell, namely, dendritic processes, axons, and nerve terminals, suggests that pp60c-src(+) may regulate pleiotropic functions in specific classes of neurons in the adult central nervous system.

Item Type: Paper
Uncontrolled Keywords: Animals Brain/*metabolism Fibroblasts/metabolism Immune Sera/immunology Immunohistochemistry *Protein Biosynthesis Proto-Oncogene Proteins/immunology/*metabolism Proto-Oncogene Proteins pp60(c-src) Rats Tissue Distribution
Subjects: organs, tissues, organelles, cell types and functions > organs types and functions > brain
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
Investigative techniques and equipment > microscopy > immunoflourescence microscopy
organism description > animal > mammal > rodent > rat
organism description > animal > mammal > rodent > rat
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: August 1990
Date Deposited: 29 Mar 2016 15:50
Last Modified: 06 Nov 2017 21:34
Related URLs:
URI: https://repository.cshl.edu/id/eprint/32300

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