McPherson, M. J., Baron, A. J., Pappin, D. J., Wootton, J. C. (November 1984) Respiratory nitrate reductase of Escherichia coli. Sequence identification of the large subunit gene. FEBS Lett, 177 (2). pp. 260-4. ISSN 0014-5793 (Print)0014-5793 (Linking)
Abstract
The nucleotide sequence is presented of part of the transcription initiation end of the nar operon of Escherichia coli K12, which encodes the respiratory nitrate reductase complex. The first coding sequence transcribed is the narG gene, encoding the large catalytic molybdoprotein of the complex. This sequence was assigned unambiguously by automated N-terminal amino acid sequencing of the purified large subunit. The deduced partial amino acid sequence of this polypeptide is hydrophilic and rich in basic residues. Membrane insertion does not involve N-terminal proteolytic processing of this subunit.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Amino Acid Sequence Base Sequence DNA Restriction Enzymes Escherichia coli/ enzymology/genetics Genes Genes, Bacterial Macromolecular Substances Nitrate Reductase Nitrate Reductases/ genetics/isolation & purification Oxygen Consumption Plasmids Species Specificity |
| Subjects: | organism description > bacteria > escherichia coli bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function |
| CSHL Authors: | |
| Communities: | CSHL labs > Pappin lab |
| Depositing User: | Matt Covey |
| Date: | 19 November 1984 |
| Date Deposited: | 10 Sep 2014 20:20 |
| Last Modified: | 10 Sep 2014 20:20 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/30779 |
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