RNA-interference-based silencing of mammalian gene expression

Conklin, D. S. (October 2003) RNA-interference-based silencing of mammalian gene expression. Chembiochem, 4 (10). pp. 1033-1039. ISSN 1439-4227

Abstract

RNA as hunter and hunted: RNA interference (RNAi) is a general method to silence gene expression. In mammals, transient delivery of dsRNA in the form of synthetic siRNAs interferes with the expression of homologous genes by causing the RISC-dependent degradation of the mRNAs. The cellular expression from DNA vectors of shRNAs, which are processed by the Dicer enzyme into siRNAs, allows the creation of continuous cell lines and transgenic animals in which suppression of a target gene is stably maintained by RNAi.

Item Type:	Paper
Uncontrolled Keywords:	antisense agents gene expression genomics RNA RNA interference DOUBLE-STRANDED-RNA FUNCTIONAL GENOMIC ANALYSIS PROTEIN-KINASE PKR VECTOR-BASED RNAI C-ELEGANS CAENORHABDITIS-ELEGANS/ MESSENGER-RNA HAIRPIN RNAS HUMAN-CELLS GERMLINE TRANSMISSION
Subjects:	bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics Investigative techniques and equipment Investigative techniques and equipment > RNAI bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNAi bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene silencing bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function organism description > animal > mammal
CSHL Authors:	Conklin, Douglas S.
Communities:	CSHL labs > Hannon lab
Depositing User:	Matt Covey
Date:	October 2003
Date Deposited:	01 Apr 2013 20:32
Last Modified:	01 Apr 2013 20:32
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/27988

Actions (login required)

Administrator's edit/view item