Conklin, D. S.
(October 2003)
RNA-interference-based silencing of mammalian gene expression.
Chembiochem, 4 (10).
pp. 1033-1039.
ISSN 1439-4227
Abstract
RNA as hunter and hunted: RNA interference (RNAi) is a general method to silence gene expression. In mammals, transient delivery of dsRNA in the form of synthetic siRNAs interferes with the expression of homologous genes by causing the RISC-dependent degradation of the mRNAs. The cellular expression from DNA vectors of shRNAs, which are processed by the Dicer enzyme into siRNAs, allows the creation of continuous cell lines and transgenic animals in which suppression of a target gene is stably maintained by RNAi.
Item Type: |
Paper
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Uncontrolled Keywords: |
antisense agents
gene expression
genomics
RNA
RNA interference
DOUBLE-STRANDED-RNA
FUNCTIONAL GENOMIC ANALYSIS
PROTEIN-KINASE PKR
VECTOR-BASED RNAI
C-ELEGANS
CAENORHABDITIS-ELEGANS/
MESSENGER-RNA
HAIRPIN RNAS
HUMAN-CELLS
GERMLINE TRANSMISSION |
Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics Investigative techniques and equipment Investigative techniques and equipment > RNAI bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNAi bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene silencing bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function organism description > animal > mammal |
CSHL Authors: |
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Communities: |
CSHL labs > Hannon lab |
Depositing User: |
Matt Covey
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Date: |
October 2003 |
Date Deposited: |
01 Apr 2013 20:32 |
Last Modified: |
01 Apr 2013 20:32 |
Related URLs: |
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URI: |
https://repository.cshl.edu/id/eprint/27988 |
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