A genetically encoded fluorescent sensor of ERK activity

Harvey, C. D., Ehrhardt, A. G., Cellurale, C., Zhong, H., Yasuda, R., Davis, R. J., Svoboda, K. (December 2008) A genetically encoded fluorescent sensor of ERK activity. Proc Natl Acad Sci U S A, 105 (49). pp. 19264-9.

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URL: http://www.ncbi.nlm.nih.gov/pubmed/19033456
DOI: 10.1073/pnas.0804598105


The activity of the ERK has complex spatial and temporal dynamics that are important for the specificity of downstream effects. However, current biochemical techniques do not allow for the measurement of ERK signaling with fine spatiotemporal resolution. We developed a genetically encoded, FRET-based sensor of ERK activity (the extracellular signal-regulated kinase activity reporter, EKAR), optimized for signal-to-noise ratio and fluorescence lifetime imaging. EKAR selectively and reversibly reported ERK activation in HEK293 cells after epidermal growth factor stimulation. EKAR signals were correlated with ERK phosphorylation, required ERK activity, and did not report the activities of JNK or p38. EKAR reported ERK activation in the dendrites and nucleus of hippocampal pyramidal neurons in brain slices after theta-burst stimuli or trains of back-propagating action potentials. EKAR therefore permits the measurement of spatiotemporal ERK signaling dynamics in living cells, including in neuronal compartments in intact tissues.

Item Type: Paper
Uncontrolled Keywords: fluorescence lifetime imaging microscopy FRET MAPK ID PROTEIN-KINASE-A SIGNAL-REGULATED KINASE LONG-TERM POTENTIATION
Subjects: Investigative techniques and equipment
Investigative techniques and equipment > microscopy > flourescence microscopy
Investigative techniques and equipment > microscopy > immunoflourescence microscopy
Investigative techniques and equipment > microscopy
CSHL Authors:
Communities: CSHL labs > Svoboda lab
School of Biological Sciences > Publications
Depositing User: Matt Covey
Date: 9 December 2008
Date Deposited: 26 Feb 2013 21:01
Last Modified: 08 Nov 2017 20:58
PMCID: PMC2614750
Related URLs:
URI: https://repository.cshl.edu/id/eprint/27535

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