Harvey, C. D., Ehrhardt, A. G., Cellurale, C., Zhong, H., Yasuda, R., Davis, R. J., Svoboda, K. (December 2008) A genetically encoded fluorescent sensor of ERK activity. Proc Natl Acad Sci U S A, 105 (49). pp. 19264-9.
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Abstract
The activity of the ERK has complex spatial and temporal dynamics that are important for the specificity of downstream effects. However, current biochemical techniques do not allow for the measurement of ERK signaling with fine spatiotemporal resolution. We developed a genetically encoded, FRET-based sensor of ERK activity (the extracellular signal-regulated kinase activity reporter, EKAR), optimized for signal-to-noise ratio and fluorescence lifetime imaging. EKAR selectively and reversibly reported ERK activation in HEK293 cells after epidermal growth factor stimulation. EKAR signals were correlated with ERK phosphorylation, required ERK activity, and did not report the activities of JNK or p38. EKAR reported ERK activation in the dendrites and nucleus of hippocampal pyramidal neurons in brain slices after theta-burst stimuli or trains of back-propagating action potentials. EKAR therefore permits the measurement of spatiotemporal ERK signaling dynamics in living cells, including in neuronal compartments in intact tissues.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | fluorescence lifetime imaging microscopy FRET MAPK ID PROTEIN-KINASE-A SIGNAL-REGULATED KINASE LONG-TERM POTENTIATION |
| Subjects: | Investigative techniques and equipment Investigative techniques and equipment > microscopy > flourescence microscopy Investigative techniques and equipment > microscopy > immunoflourescence microscopy Investigative techniques and equipment > microscopy |
| CSHL Authors: | |
| Communities: | CSHL labs > Svoboda lab School of Biological Sciences > Publications |
| Depositing User: | Matt Covey |
| Date: | 9 December 2008 |
| Date Deposited: | 26 Feb 2013 21:01 |
| Last Modified: | 08 Nov 2017 20:58 |
| PMCID: | PMC2614750 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/27535 |
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