Isolation of the Rb-related p130 through its interaction with CDK2 and cyclins

Hannon, G. J., Demetrick, D., Beach, D. (1993) Isolation of the Rb-related p130 through its interaction with CDK2 and cyclins. Genes and Development, 7 (12 A). pp. 2378-2391. ISSN 08909369 (ISSN)

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Abstract

A two-hybrid protein interaction screen was used to isolate cDNAs encoding human proteins that can interact with human CDK2 in yeast. A new member of the retinoblastoma susceptibility gene family, Rbr-2 (Rb-related), was obtained. The sequence of the Rbr-2 protein shares ~50% identity with p107 and homology to Rb within the pocket domain. Several lines of evidence indicate that Rbr-2 is the adenovirus E1A-associated p130. Like Rb and p107, p130(Rbr-2) can bind to viral oncoproteins, SV40 large T antigen, and adenovirus E1A through its pocket domain. Although p130(Rbr-2) does not bind to CDK2 in vitro, it can interact with cyclins, with a clear preference for D-type cyclins. Because both CDK2 and p130(Rbr-2) show affinity for cyclins, we suggest that p130(Rbr-2) and CDK2 interacted through a yeast-derived cyclin bridge in the two-hybrid screen. The gene encoding p130(Rbr-2) mapped to 16q13, a region of frequent genomic alteration in human tumors.

Item Type:	Paper
Uncontrolled Keywords:	cycline hybrid protein oncoprotein retinoblastoma protein virus protein animal cell antigen binding article dna library fluorescence gene location genetic susceptibility immunoprecipitation in situ hybridization multigene family nonhuman priority journal protein analysis protein binding protein isolation protein protein interaction sequence homology yeast Adenovirus E1A Proteins Amino Acid Sequence Antigens, Polyomavirus Transforming Base Sequence Chromosome Mapping Cyclins Human Molecular Sequence Data Protein Kinases Proteins Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S.
Subjects:	organs, tissues, organelles, cell types and functions > cell types and functions > cell functions organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell regulation organs, tissues, organelles, cell types and functions > cell types and functions
CSHL Authors:	Beach, David H. Hannon, Gregory J.
Communities:	CSHL labs > Hannon lab
Depositing User:	Matt Covey
Date:	1993
Date Deposited:	09 Jan 2013 17:36
Last Modified:	14 Jan 2013 21:52
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/26496

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