MicroRNA-dependent localization of targeted mRNAs to mammalian P-bodies

Liu, J., Valencia-Sanchez, M. A., Hannon, G. J., Parker, R. (July 2005) MicroRNA-dependent localization of targeted mRNAs to mammalian P-bodies. Nature Cell Biology, 7 (7). pp. 719-23. ISSN 1465-7392

Abstract

Small RNAs, including small interfering RNAs (siRNAs) and microRNAs (miRNAs) can silence target genes through several different effector mechanisms. Whereas siRNA-directed mRNA cleavage is increasingly understood, the mechanisms by which miRNAs repress protein synthesis are obscure. Recent studies have revealed the existence of specific cytoplasmic foci, referred to herein as processing bodies (P-bodies), which contain untranslated mRNAs and can serve as sites of mRNA degradation. Here we demonstrate that Argonaute proteins--the signature components of the RNA interference (RNAi) effector complex, RISC--localize to mammalian P-bodies. Moreover, reporter mRNAs that are targeted for translational repression by endogenous or exogenous miRNAs become concentrated in P-bodies in a miRNA-dependent manner. These results provide a link between miRNA function and mammalian P-bodies and suggest that translation repression by RISC delivers mRNAs to P-bodies, either as a cause or as a consequence of inhibiting protein synthesis.

Item Type:	Paper
Uncontrolled Keywords:	3 Untranslated Regions genetics metabolism Caenorhabditis elegans Proteins genetics Cell Line Cell Line Tumor Cytoplasmic Structures metabolism Endoribonucleases metabolism Gene Expression Regulation Hela Cells Humans Immunoprecipitation MicroRNAs genetics metabolism Microscopy Fluorescence Mutation physiology Peptide Initiation Factors genetics metabolism Protein Binding RNA Transport RNA Messenger genetics metabolism RNA Small Interfering genetics metabolism RNA Induced Silencing Complex metabolism Receptors CXCR4 genetics Trans Activators metabolism Transfection
Subjects:	bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNAi bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > argonaute proteins bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mRNA dynamics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > sRNA
CSHL Authors:	Hannon, Gregory J. Liu, Jidong
Communities:	CSHL labs > Hannon lab
Depositing User:	Editor Margaret Fantz
Date:	July 2005
Date Deposited:	16 Apr 2012 18:29
Last Modified:	01 Mar 2013 20:16
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/26115

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