Liu, J., Valencia-Sanchez, M. A., Hannon, G. J., Parker, R.
(July 2005)
MicroRNA-dependent localization of targeted mRNAs to mammalian P-bodies.
Nature Cell Biology, 7 (7).
pp. 719-23.
ISSN 1465-7392
Abstract
Small RNAs, including small interfering RNAs (siRNAs) and microRNAs (miRNAs) can silence target genes through several different effector mechanisms. Whereas siRNA-directed mRNA cleavage is increasingly understood, the mechanisms by which miRNAs repress protein synthesis are obscure. Recent studies have revealed the existence of specific cytoplasmic foci, referred to herein as processing bodies (P-bodies), which contain untranslated mRNAs and can serve as sites of mRNA degradation. Here we demonstrate that Argonaute proteins--the signature components of the RNA interference (RNAi) effector complex, RISC--localize to mammalian P-bodies. Moreover, reporter mRNAs that are targeted for translational repression by endogenous or exogenous miRNAs become concentrated in P-bodies in a miRNA-dependent manner. These results provide a link between miRNA function and mammalian P-bodies and suggest that translation repression by RISC delivers mRNAs to P-bodies, either as a cause or as a consequence of inhibiting protein synthesis.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
3 Untranslated Regions genetics metabolism
Caenorhabditis elegans Proteins genetics
Cell Line
Cell Line Tumor
Cytoplasmic Structures metabolism
Endoribonucleases metabolism
Gene Expression Regulation
Hela Cells
Humans
Immunoprecipitation
MicroRNAs genetics metabolism
Microscopy Fluorescence
Mutation physiology
Peptide Initiation Factors genetics metabolism
Protein Binding
RNA Transport
RNA Messenger genetics metabolism
RNA Small Interfering genetics metabolism
RNA Induced Silencing Complex metabolism
Receptors CXCR4 genetics
Trans Activators metabolism
Transfection |
| Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNAi
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > argonaute proteins
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mRNA dynamics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > sRNA |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Hannon lab |
| Depositing User: |
Editor Margaret Fantz
|
| Date: |
July 2005 |
| Date Deposited: |
16 Apr 2012 18:29 |
| Last Modified: |
01 Mar 2013 20:16 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/26115 |
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