Exchange of GATA Factors Mediates Transitions in Looped Chromatin Organization at a Developmentally Regulated Gene Locus

Jing, H., Vakoc, C. R., Ying, L., Mandat, S., Wang, H., Zheng, X., Blobel, G. A. (2008) Exchange of GATA Factors Mediates Transitions in Looped Chromatin Organization at a Developmentally Regulated Gene Locus. Molecular Cell, 29 (2). pp. 232-242. ISSN 10972765 (ISSN)

Abstract

Enhancers can regulate designate promoters over long distances by forming chromatin loops. Whether chromatin loops are lost or reconfigured during gene repression is largely unexplored. We examined the chromosome conformation of the Kit gene that is expressed during early erythropoiesis but is downregulated upon cell maturation. Kit expression is controlled by sequential occupancy of two GATA family transcription factors. In immature cells, a distal enhancer bound by GATA-2 is in physical proximity with the active Kit promoter. Upon cell maturation, GATA-1 displaces GATA-2 and triggers a loss of the enhancer/promoter interaction. Moreover, GATA-1 reciprocally increases the proximity in nuclear space among distinct downstream GATA elements. GATA-1-induced transitions in chromatin conformation are not simply the consequence of transcription inhibition and require the cofactor FOG-1. This work shows that a GATA factor exchange reconfigures higher-order chromatin organization, and suggests that de novo chromatin loop formation is employed by nuclear factors to specify repressive outcomes. © 2008 Elsevier Inc. All rights reserved.

Item Type:	Paper
Uncontrolled Keywords:	DNA transcription factor GATA transcription factor GATA 1 transcription factor GATA 2 article cell maturation chromatin structure chromosome conformation controlled study down regulation erythropoiesis gene expression gene locus gene sequence protein depletion protein protein interaction Animals Cell Line Chromatin Chromatin Assembly and Disassembly Enhancer Elements (Genetics) GATA1 Transcription Factor GATA2 Transcription Factor Gene Silencing Mice Nuclear Proteins Proto-Oncogene Proteins c-kit Quantitative Trait Loci Response Elements Transcription Factors
Subjects:	bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor
CSHL Authors:	Vakoc, Christopher R.
Communities:	CSHL labs > Vakoc lab
Depositing User:	CSHL Librarian
Date:	2008
Date Deposited:	19 Mar 2012 19:32
Last Modified:	13 Mar 2013 20:23
PMCID:	PMC2254447
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/25394

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