Jing, H., Vakoc, C. R., Ying, L., Mandat, S., Wang, H., Zheng, X., Blobel, G. A.
(2008)
Exchange of GATA Factors Mediates Transitions in Looped Chromatin Organization at a Developmentally Regulated Gene Locus.
Molecular Cell, 29 (2).
pp. 232-242.
ISSN 10972765 (ISSN)
Abstract
Enhancers can regulate designate promoters over long distances by forming chromatin loops. Whether chromatin loops are lost or reconfigured during gene repression is largely unexplored. We examined the chromosome conformation of the Kit gene that is expressed during early erythropoiesis but is downregulated upon cell maturation. Kit expression is controlled by sequential occupancy of two GATA family transcription factors. In immature cells, a distal enhancer bound by GATA-2 is in physical proximity with the active Kit promoter. Upon cell maturation, GATA-1 displaces GATA-2 and triggers a loss of the enhancer/promoter interaction. Moreover, GATA-1 reciprocally increases the proximity in nuclear space among distinct downstream GATA elements. GATA-1-induced transitions in chromatin conformation are not simply the consequence of transcription inhibition and require the cofactor FOG-1. This work shows that a GATA factor exchange reconfigures higher-order chromatin organization, and suggests that de novo chromatin loop formation is employed by nuclear factors to specify repressive outcomes. © 2008 Elsevier Inc. All rights reserved.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
DNA
transcription factor GATA
transcription factor GATA 1
transcription factor GATA 2
article
cell maturation
chromatin structure
chromosome
conformation
controlled study
down regulation
erythropoiesis
gene expression
gene locus
gene sequence
protein depletion
protein protein interaction
Animals
Cell Line
Chromatin
Chromatin Assembly and Disassembly
Enhancer Elements (Genetics)
GATA1 Transcription Factor
GATA2 Transcription Factor
Gene Silencing
Mice
Nuclear Proteins
Proto-Oncogene Proteins c-kit
Quantitative Trait Loci
Response Elements
Transcription Factors |
| Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Vakoc lab |
| Depositing User: |
CSHL Librarian
|
| Date: |
2008 |
| Date Deposited: |
19 Mar 2012 19:32 |
| Last Modified: |
13 Mar 2013 20:23 |
| PMCID: |
PMC2254447 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/25394 |
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