Kurita, T., Cunha, G. R., Robboy, S. J., Mills, A. A., Medina, R. T. (September 2005) Differential expression of p63 isoforms in female reproductive organs. Mechanisms of Development, 122 (9). pp. 1043-1055. ISSN 0925-4773
Abstract
p63 is the identity switch for uterine/vaginal epithelial cell fate, and disruption of p63 expression by diethylstilbestrol (DES) induces cervical/vaginal adenosis in mice. In this article, we report the expression patterns of p63 isofonns (TA, Delta N, alpha, beta and gamma) in mice, focusing on the reproductive tract. We also present the reproductive tract phenotype of female p63(-/-) mice. Finally, to better evaluate the potential role of p63 in human development of DES-induced cervical/vaginal adenosis, we describe the ontogeny of p63 in human female fetuses. In adult mice, the Delta N isoforms of p63 were expressed only in squamous/basal/myoepithelial cells of epithelial tissues, while TA isoforms of p63 were highly expressed in germ cells of the ovary and testis. In fetal mice, the Delta N and alpha forms of p63 were expressed in the cloacal and urogenital sinus epithelia. In the female p63(-/-) mice, the sinus vagina developed, but p63(-/-) sinus vaginal epithelium failed to undergo squamous differentiation confirming an essential role of p63 in squamous epithelial differentiation. Although TAp63 was highly expressed in developing primordial germ cells/oocytes, p63(-/-) ovaries and oocytes developed normally. The ontogeny of p63 in female reproductive organs was essentially identical in mouse and human. In the human fetus at the susceptible stage for DES-induced cervical/vaginal adenosis, most cervical/vaginal epithelial cells were columnar and negative for p63. Therefore, inhibition of p63 expression by DES should change the cell fate of human Mullerian duct epithelial cells and cause cervical/vaginal adenosis as previously demonstrated in mouse. (c) 2005 Elsevier Ireland Ltd. All rights reserved.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | squamous epithelium basal cell germ cell uterus vagina oviduct ovary prostate mammary gland testis vaginal adenosis mullerian duct young woman P53 homolog diethylsilbesterol mouse CELLS adenocarcinoma progesterone estrogen DES TA |
| Subjects: | bioinformatics > genomics and proteomics > annotation > gene expression profiling annotation bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > p63 |
| CSHL Authors: | |
| Communities: | CSHL labs > Mills lab |
| Depositing User: | CSHL Librarian |
| Date: | September 2005 |
| Date Deposited: | 09 Jan 2012 18:57 |
| Last Modified: | 02 Feb 2017 19:57 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/22626 |
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