Chang, K., Marran, K., Valentine, A., Hannon, G. J. (2013) Infection of mammalian cells with retroviral shRNAs. Cold Spring Harbor Protocols, 2013 (8). pp. 732-733. ISSN 19403402
Abstract
Viral infection is a quite simple approach for stably introducing transgenes (e.g., those encoding shorthairpin RNAs [shRNAs]) into the genome. The critical aspects are that the virus and the target cell should be appropriately matched. For example, a virus bearing an ecotropic envelope protein will not infect a human cell line unless the appropriate receptor has been purposefully expressed. VSV-G (vesicular stomatitus virus glycoprotein) pseudotyped viruses have the greatest host range. Nondividing cells can only be infected with lentiviruses, but the additional safety precautions necessary for the use of these tools should dissuade their application to routinely cultured cell lines.
| Item Type: | Paper |
|---|---|
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > non-coding RNA organism description > virus > retrovirus |
| CSHL Authors: | |
| Communities: | CSHL labs > Chang lab CSHL labs > Hannon lab |
| Depositing User: | Adrian Gomez |
| Date: | 2013 |
| Date Deposited: | 22 Nov 2019 20:29 |
| Last Modified: | 22 Nov 2019 20:29 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/38726 |
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