An Efficient Cell Sorting Protocol for Maize Protoplasts

Ortiz-Ramirez, C., Arevalo, E. D., Xu, X., Jackson, D. P., Birnbaum, K. D. (August 2018) An Efficient Cell Sorting Protocol for Maize Protoplasts. Curr Protoc Plant Biol, 3 (3). e20072.

URL: https://www.ncbi.nlm.nih.gov/pubmed/30138552
DOI: 10.1002/cppb.20072

Abstract

Maize is one the most widely cultivated crops worldwide and an important model system for the study of genetics and cytogenetics. Although the availability of a genome sequence has enabled new quantitative genomic studies, developing methods to isolate specific types of cells will enable useful approaches for transcriptomic analysis in the crop plant. Fluorescence-activated cell sorting (FACS) is a powerful technique for cell isolation and the study of transcriptional profiles from specific cell populations. The use of FACS on plant cells requires the generation of protoplasts by tissue digestion and cell wall removal. Although some protocols are available, they mainly focus on dicot species and obtaining sufficient protoplasts from inner tissue layers has been challenging in both monocots and dicots. Here, we report a new protocol that dramatically increases protoplast yield from maize for subsequent cell isolation by FACS. This protocol is efficient in generating protoplasts from root and shoot inner layers and can also be applied successfully to Arabidopsis thaliana. (c) 2018 by John Wiley & Sons, Inc.

Item Type: Paper
Subjects: organism description > plant > maize
CSHL Authors:
Depositing User: Matthew Dunn
Date: 23 August 2018
Date Deposited: 28 Aug 2018 18:09
Last Modified: 20 Feb 2019 21:17
PMCID: PMC6135669
Related URLs:
URI: http://repository.cshl.edu/id/eprint/37165

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving