Paracrine brassinosteroid signaling at the stem cell niche controls cellular regeneration

Lozano-Elena, F., Planas-Riverola, A., Vilarrasa-Blasi, J., Schwab, R., Cano-Delgado, A. I. (January 2018) Paracrine brassinosteroid signaling at the stem cell niche controls cellular regeneration. J Cell Sci, 131 (2). jcs204065. ISSN 0021-9533

URL: https://www.ncbi.nlm.nih.gov/pubmed/29242230
DOI: 10.1242/jcs.204065

Abstract

Stem cell regeneration is crucial for both cell turnover and tissue healing in multicellular organisms. In Arabidopsis roots, a reduced group of cells known as the quiescent center (QC) acts as a cell reservoir for surrounding stem cells during both normal growth and in response to external damage. Although cells of the QC have a very low mitotic activity, plant hormones such as brassinosteroids (BR) can promote QC divisions. Here, we used a tissue-specific strategy to investigate the spatial signaling requirements of BR-mediated QC divisions. We generated stem cell niche-specific receptor knockout lines by placing an artificial microRNA against BRI1 (BRASSINOSTEROID RESPONSE INSENSITIVE 1) under the control of the QC-specific promoter WOX5. Additionally, QC-specific knock-in lines for BRI1 and its downstream transcription factor BES1 (BRI1-EMS-SUPPRESOR1) were also created using the WOX5 promoter. By analyzing the roots of these lines, we uncover that BES1-mediated signaling cell-autonomously promotes QC divisions, that BRI1 is essential for sensing nearby inputs and triggering QC divisions, and that DNA damage promotes BR-dependent paracrine signaling in the stem cell niche as a prerequisite to stem cell replenishment.

Item Type: Paper
Uncontrolled Keywords: Brassinosteroid Cell division DNA damage Paracrine Quiescent center Stem cell
Subjects: organism description > plant > Arabidopsis
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell division
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > stem cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > stem cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > stem cells
CSHL Authors:
Communities: CSHL labs > Martienssen lab
Depositing User: Matt Covey
Date: 29 January 2018
Date Deposited: 20 Dec 2017 16:22
Last Modified: 07 Mar 2018 14:14
PMCID: PMC5818034
Related URLs:
URI: http://repository.cshl.edu/id/eprint/35731

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