Lozano-Elena, F., Planas-Riverola, A., Vilarrasa-Blasi, J., Schwab, R., Cano-Delgado, A. I. (January 2018) Paracrine brassinosteroid signaling at the stem cell niche controls cellular regeneration. J Cell Sci, 131 (2). jcs204065. ISSN 0021-9533
Abstract
Stem cell regeneration is crucial for both cell turnover and tissue healing in multicellular organisms. In Arabidopsis roots, a reduced group of cells known as the quiescent center (QC) acts as a cell reservoir for surrounding stem cells during both normal growth and in response to external damage. Although cells of the QC have a very low mitotic activity, plant hormones such as brassinosteroids (BR) can promote QC divisions. Here, we used a tissue-specific strategy to investigate the spatial signaling requirements of BR-mediated QC divisions. We generated stem cell niche-specific receptor knockout lines by placing an artificial microRNA against BRI1 (BRASSINOSTEROID RESPONSE INSENSITIVE 1) under the control of the QC-specific promoter WOX5. Additionally, QC-specific knock-in lines for BRI1 and its downstream transcription factor BES1 (BRI1-EMS-SUPPRESOR1) were also created using the WOX5 promoter. By analyzing the roots of these lines, we uncover that BES1-mediated signaling cell-autonomously promotes QC divisions, that BRI1 is essential for sensing nearby inputs and triggering QC divisions, and that DNA damage promotes BR-dependent paracrine signaling in the stem cell niche as a prerequisite to stem cell replenishment.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Brassinosteroid Cell division DNA damage Paracrine Quiescent center Stem cell |
| Subjects: | organism description > plant > Arabidopsis organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell division organs, tissues, organelles, cell types and functions > cell types and functions > cell types > stem cells organs, tissues, organelles, cell types and functions > cell types and functions > cell types > stem cells organs, tissues, organelles, cell types and functions > cell types and functions > cell types > stem cells |
| CSHL Authors: | |
| Communities: | CSHL labs > Martienssen lab |
| Depositing User: | Matt Covey |
| Date: | 29 January 2018 |
| Date Deposited: | 20 Dec 2017 16:22 |
| Last Modified: | 07 Mar 2018 14:14 |
| PMCID: | PMC5818034 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/35731 |
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