Transplantation of Beta-Cells from Transgenic Mice into Nude Athymic Diabetic Rats Restores Glucose Regulation

Hicks, B. A., Stein, R., Efrat, S., Grant, S., Hanahan, D., Demetriou, A. A. (1991) Transplantation of Beta-Cells from Transgenic Mice into Nude Athymic Diabetic Rats Restores Glucose Regulation. Diabetes Research and Clinical Practice, 14 (3). pp. 157-164. ISSN 0168-8227

DOI: 10.1016/0168-8227(91)90016-7


We have shown that elevated plasma D-glucose levels in experimentally-induced diabetic nude athymic rats can be reduced by intraperitoneal transplantation of microcarrier-attached insulin producing beta-cells from the mouse pancreatic beta-cell line, beta-TC-1. The reduction in the level of hyperglycemia was observed as early as two days following cell transplantation and was associated with a concomitant increase in plasma insulin levels. beta-TC-1 cell transplanted diabetic rats had plasma D-glucose levels similar to those found in non-diabetic control animals and remained normoglycemic throughout the 39 day experimental period. The beta-TC-1 cell transplanted diabetic rats also had near normalization of body weight, food and water intake and of urine output when compared to control diabetic and non-diabetic rats. Similarly, they exhibited improved blood glucose clearance following intravenous D-glucose administration. These results suggest that beta-TC-1 cells regulate D-glucose homeostasis following transplantation into diabetic rat recipients in a manner similar to that of endogenous pancreatic beta-cells.

Item Type: Paper
Uncontrolled Keywords: beta-cell transplantation streptozotocin induced diabetes insulin-dependent diabetes-mellitus cell therapy rat pancreas transplantation hyperglycemia registry islets
Subjects: diseases & disorders > nutritional and metabolic diseases > diabetes
organism description > animal > mammal > rodent > mouse
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > transgenic animal
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date Deposited: 09 Dec 2015 20:46
Last Modified: 09 Dec 2015 20:46
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