Telomerase primer specificity and chromosome healing

Harrington, L. A., Greider, C. W. (October 1991) Telomerase primer specificity and chromosome healing. Nature, 353 (6343). pp. 451-4. ISSN 0028-0836 (Print)0028-0836 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/1896088
DOI: 10.1038/353451a0

Abstract

Chromosome healing by de novo telomere addition at nontelomeric sites has been well characterized in several organisms. The Tetrahymena telomerase ribonucleoprotein uses an internal RNA template to catalyse d(TTGGGG)n telomere addition to the 3' end of telomeric sequence in vitro and in vivo. Studies of telomerase RNA indicated that hybridization of the RNA template region, 5'-CAACCCCAA-3', to the 3' end of single-stranded telomeric oligonucleotides might be important for primer recognition and utilization. The apparent requirement of telomerase for pre-existing telomeric sequence has raised questions regarding its role in chromosome healing. We report here that Tetrahymena telomerase can specifically elongate single-stranded DNA oligonucleotides whose termini are not complementary to the RNA template sequence 5'-CAACCCCAA-3'. These data suggest that telomerase may be able to heal chromosomes directly in vivo.

Item Type: Paper
Uncontrolled Keywords: Animals Base Sequence Chromosomes/*metabolism DNA Nucleotidylexotransferase/*metabolism DNA, Protozoan/metabolism DNA, Single-Stranded/*metabolism Models, Genetic Molecular Sequence Data Nucleic Acid Hybridization Oligodeoxyribonucleotides/*metabolism RNA, Protozoan/metabolism Substrate Specificity Tetrahymena/enzymology/genetics
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > telomerase
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > telomeres
CSHL Authors:
Communities: CSHL labs > Greider lab
Depositing User: Matt Covey
Date: 3 October 1991
Date Deposited: 10 Dec 2015 15:00
Last Modified: 10 Dec 2015 15:00
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32158

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