Harrington, L. A., Greider, C. W.
(October 1991)
Telomerase primer specificity and chromosome healing.
Nature, 353 (6343).
pp. 451-4.
ISSN 0028-0836 (Print)0028-0836 (Linking)
Abstract
Chromosome healing by de novo telomere addition at nontelomeric sites has been well characterized in several organisms. The Tetrahymena telomerase ribonucleoprotein uses an internal RNA template to catalyse d(TTGGGG)n telomere addition to the 3' end of telomeric sequence in vitro and in vivo. Studies of telomerase RNA indicated that hybridization of the RNA template region, 5'-CAACCCCAA-3', to the 3' end of single-stranded telomeric oligonucleotides might be important for primer recognition and utilization. The apparent requirement of telomerase for pre-existing telomeric sequence has raised questions regarding its role in chromosome healing. We report here that Tetrahymena telomerase can specifically elongate single-stranded DNA oligonucleotides whose termini are not complementary to the RNA template sequence 5'-CAACCCCAA-3'. These data suggest that telomerase may be able to heal chromosomes directly in vivo.
| Item Type: |
Paper
|
| Uncontrolled Keywords: |
Animals
Base Sequence
Chromosomes/*metabolism
DNA Nucleotidylexotransferase/*metabolism
DNA, Protozoan/metabolism
DNA, Single-Stranded/*metabolism
Models, Genetic
Molecular Sequence Data
Nucleic Acid Hybridization
Oligodeoxyribonucleotides/*metabolism
RNA, Protozoan/metabolism
Substrate Specificity
Tetrahymena/enzymology/genetics |
| Subjects: |
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > telomerase
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > telomeres |
| CSHL Authors: |
|
| Communities: |
CSHL labs > Greider lab |
| Depositing User: |
Matt Covey
|
| Date: |
3 October 1991 |
| Date Deposited: |
10 Dec 2015 15:00 |
| Last Modified: |
10 Dec 2015 15:00 |
| Related URLs: |
|
| URI: |
https://repository.cshl.edu/id/eprint/32158 |
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