Simian virus 40 large-T antigen expresses a biological activity complementary to the p300-associated transforming function of the adenovirus E1A gene products

Yaciuk, P., Carter, M. C., Pipas, J. M., Moran, E. (1991) Simian virus 40 large-T antigen expresses a biological activity complementary to the p300-associated transforming function of the adenovirus E1A gene products. Mol Cell Biol, 11 (4). pp. 2116-24. ISSN 0270-7306 (Print)0270-7306 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/1848672
DOI: 10.1128/MCB.11.4.2116

Abstract

In this report we present evidence that simian virus 40 T antigen encodes a biological activity that is functionally equivalent to the transforming activity lost by deletion of the E1A p300-binding region. T-antigen constructs from which the pRb-binding region has been deleted are virtually unable to induce foci of transformed cells in a ras cooperation assay in primary baby rat kidney cells. Nevertheless, such a construct can cooperate with an E1A N-terminal deletion mutant, itself devoid of transforming activity, to induce foci in this assay. The heterologous trans-cooperating activity observed between E1A and T-antigen deletion products is as efficient as trans cooperation between mutants expressing individual E1A domains. The cooperating function can be impaired by a deletion near the N terminus of T antigen. Such a deletion impairs neither the p53-binding function nor the activity of the pRb-binding region.

Item Type: Paper
Uncontrolled Keywords: Adenovirus Early Proteins Animals Antigens, Polyomavirus Transforming/genetics/*metabolism Cell Line Cell Transformation, Viral Cloning, Molecular Gene Expression Genetic Complementation Test Mutation Oncogene Proteins, Viral/genetics/*metabolism Precipitin Tests Rats Simian virus 40/*immunology
Subjects: organism description > virus > adenovirus
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
organism description > virus > viral transfection
organism description > virus
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date Deposited: 10 Dec 2015 19:34
Last Modified: 10 Dec 2015 19:34
PMCID: PMC359899
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32144

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