Yaciuk, P., Carter, M. C., Pipas, J. M., Moran, E. (April 1991) Simian virus 40 large-T antigen expresses a biological activity complementary to the p300-associated transforming function of the adenovirus E1A gene products. Mol Cell Biol, 11 (4). pp. 2116-24. ISSN 0270-7306 (Print)0270-7306 (Linking)
Abstract
In this report we present evidence that simian virus 40 T antigen encodes a biological activity that is functionally equivalent to the transforming activity lost by deletion of the E1A p300-binding region. T-antigen constructs from which the pRb-binding region has been deleted are virtually unable to induce foci of transformed cells in a ras cooperation assay in primary baby rat kidney cells. Nevertheless, such a construct can cooperate with an E1A N-terminal deletion mutant, itself devoid of transforming activity, to induce foci in this assay. The heterologous trans-cooperating activity observed between E1A and T-antigen deletion products is as efficient as trans cooperation between mutants expressing individual E1A domains. The cooperating function can be impaired by a deletion near the N terminus of T antigen. Such a deletion impairs neither the p53-binding function nor the activity of the pRb-binding region.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Adenovirus Early Proteins Animals Antigens, Polyomavirus Transforming/genetics/*metabolism Cell Line Cell Transformation, Viral Cloning, Molecular Gene Expression Genetic Complementation Test Mutation Oncogene Proteins, Viral/genetics/*metabolism Precipitin Tests Rats Simian virus 40/*immunology |
| Subjects: | organism description > virus > adenovirus bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression organism description > virus > viral transfection organism description > virus |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Matt Covey |
| Date: | April 1991 |
| Date Deposited: | 10 Dec 2015 19:34 |
| Last Modified: | 10 Dec 2015 19:34 |
| PMCID: | PMC359899 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/32144 |
Actions (login required)
 |
Administrator's edit/view item |