Genome and transcriptome of the regeneration-competent flatworm, Macrostomum lignano

Wasik, K., Gurtowski, J., Zhou, X., Ramos, O. M., Delas, M. J., Battistoni, G., El Demerdash, O., Falciatori, I., Vizoso, D. B., Smith, A. D., Ladurner, P., Scharer, L., McCombie, W. R., Hannon, G. J., Schatz, M. (2015) Genome and transcriptome of the regeneration-competent flatworm, Macrostomum lignano. Proc Natl Acad Sci U S A, 112 (40). pp. 12462-12467. ISSN 1091-6490 (Electronic)0027-8424 (Linking)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/26392545
DOI: 10.1073/pnas.1516718112

Abstract

The free-living flatworm, Macrostomum lignano has an impressive regenerative capacity. Following injury, it can regenerate almost an entirely new organism because of the presence of an abundant somatic stem cell population, the neoblasts. This set of unique properties makes many flatworms attractive organisms for studying the evolution of pathways involved in tissue self-renewal, cell-fate specification, and regeneration. The use of these organisms as models, however, is hampered by the lack of a well-assembled and annotated genome sequences, fundamental to modern genetic and molecular studies. Here we report the genomic sequence of M. lignano and an accompanying characterization of its transcriptome. The genome structure of M. lignano is remarkably complex, with approximately 75% of its sequence being comprised of simple repeats and transposon sequences. This has made high-quality assembly from Illumina reads alone impossible (N50 = 222 bp). We therefore generated 130x coverage by long sequencing reads from the Pacific Biosciences platform to create a substantially improved assembly with an N50 of 64 Kbp. We complemented the reference genome with an assembled and annotated transcriptome, and used both of these datasets in combination to probe gene-expression patterns during regeneration, examining pathways important to stem cell function.

Item Type: Paper
Uncontrolled Keywords: Macrostomum flatworm genome neoblast regeneration
Subjects: organism description > animal > flatworm
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > genomes
organism description > animal > flatworm > Macrostomum lignano
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > transcriptomes
CSHL Authors:
Communities: CSHL labs > Hannon lab
CSHL labs > McCombie lab
CSHL labs > Schatz lab
Watson School > Publications
Depositing User: Matt Covey
Date Deposited: 01 Oct 2015 22:01
Last Modified: 08 Nov 2017 20:11
PMCID: PMC4603488
Related URLs:
URI: http://repository.cshl.edu/id/eprint/31897

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