Testing the in vivo role of protein kinase C and c-fos in neurite outgrowth by microinjection of antibodies into PC12 cells

Altin, J. G., Wetts, R., Riabowol, K. T., Bradshaw, R. A. (March 1992) Testing the in vivo role of protein kinase C and c-fos in neurite outgrowth by microinjection of antibodies into PC12 cells. Molecular Biology of the Cell, 3 (3). pp. 323-333. ISSN 1059-1524

URL: http://www.ncbi.nlm.nih.gov/pubmed/1627832
DOI: 10.1091/mbc.3.3.323

Abstract

To define the molecular bases of growth factor-induced signal transduction pathways, antibodies known to block the activity of either protein kinase C (PKC) or the fos protein were introduced into PC12 cells by microinjection. The antibody against PKC significantly inhibited neurite outgrowth when scored 24 h after microinjection and exposure to nerve growth factor (NGF). Microinjection of antibodies to fos significantly increased the percentage of neurite-bearing cells after exposure to either NGF or basic fibroblast growth factor (bFGF) but inhibited the stimulation of DNA synthesis by serum, suggesting that in PC12 cells, fos is involved in cellular proliferation. Thus, activation of PKC is involved in the induction of neurite outgrowth by NGF, but expression of the fos protein, which is induced by both NGF and bFGF, is not necessary and inhibits neurite outgrowth.

Item Type: Paper
Uncontrolled Keywords: NERVE GROWTH-FACTOR PC12 PHEOCHROMOCYTOMA CELLS INDUCED NEURONAL DIFFERENTIATION HA-RAS ONCOGENE V-SRC MORPHOLOGICAL-DIFFERENTIATION GENE-EXPRESSION INDUCTION PHOSPHORYLATION TRANSCRIPTION
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > fibroblast growth factor
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > neurogenesis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase > Protein kinase C
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: March 1992
Date Deposited: 25 Sep 2015 19:18
Last Modified: 25 Sep 2015 19:18
PMCID: PMC275533
Related URLs:
URI: http://repository.cshl.edu/id/eprint/31830

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