Altin, J. G., Wetts, R., Riabowol, K. T., Bradshaw, R. A. (March 1992) Testing the in vivo role of protein kinase C and c-fos in neurite outgrowth by microinjection of antibodies into PC12 cells. Molecular Biology of the Cell, 3 (3). pp. 323-333. ISSN 1059-1524
Abstract
To define the molecular bases of growth factor-induced signal transduction pathways, antibodies known to block the activity of either protein kinase C (PKC) or the fos protein were introduced into PC12 cells by microinjection. The antibody against PKC significantly inhibited neurite outgrowth when scored 24 h after microinjection and exposure to nerve growth factor (NGF). Microinjection of antibodies to fos significantly increased the percentage of neurite-bearing cells after exposure to either NGF or basic fibroblast growth factor (bFGF) but inhibited the stimulation of DNA synthesis by serum, suggesting that in PC12 cells, fos is involved in cellular proliferation. Thus, activation of PKC is involved in the induction of neurite outgrowth by NGF, but expression of the fos protein, which is induced by both NGF and bFGF, is not necessary and inhibits neurite outgrowth.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | NERVE GROWTH-FACTOR PC12 PHEOCHROMOCYTOMA CELLS INDUCED NEURONAL DIFFERENTIATION HA-RAS ONCOGENE V-SRC MORPHOLOGICAL-DIFFERENTIATION GENE-EXPRESSION INDUCTION PHOSPHORYLATION TRANSCRIPTION |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > fibroblast growth factor organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > neurogenesis bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase > Protein kinase C |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Matt Covey |
| Date: | March 1992 |
| Date Deposited: | 25 Sep 2015 19:18 |
| Last Modified: | 25 Sep 2015 19:18 |
| PMCID: | PMC275533 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/31830 |
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