cdt1 is an essential target of the Cdc10/Sct1 transcription factor: requirement for DNA replication and inhibition of mitosis

Hofmann, J. F., Beach, D. (1994) cdt1 is an essential target of the Cdc10/Sct1 transcription factor: requirement for DNA replication and inhibition of mitosis. Embo J, 13 (2). pp. 425-34. ISSN 0261-4189 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/8313888

Abstract

We have used an immunoprecipitation-PCR cycle to isolate physically genomic DNA sequences that are bound by the fission yeast cdc10 gene product in an attempt to identify novel target genes. An essential gene, cdt1, has been isolated whose expression is cell cycle regulated in a cdc10 dependent manner. The cdt1 promoter contains a recognition site for a sequence specific DNA binding factor. The cdc10 gene product is a component of this factor. Ectopic expression of cdt1 can complement a temperature sensitive mutation of cdc10 at semipermissive temperature. Cells carrying a null allele of cdt1 are defective in DNA replication but initiate mitotic events, suggesting that cdt1 is essential for the normal dependency relationship of S-phase and mitosis.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Base Sequence Binding Sites Cell Cycle Proteins/ metabolism Cloning, Molecular DNA Replication DNA, Fungal DNA-Binding Proteins/ metabolism Fungal Proteins/ metabolism Genetic Complementation Test Mitosis/ genetics Molecular Sequence Data Phenotype Promoter Regions (Genetics) Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Schizosaccharomyces/genetics Schizosaccharomyces pombe Proteins Temperature Transcription Factors/ metabolism
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
organs, tissues, organelles, cell types and functions > organelles, types and functions > mitosis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA expression > promoter
CSHL Authors:
Communities: CSHL labs > Beach lab
Depositing User: Matt Covey
Date Deposited: 04 May 2015 19:21
Last Modified: 04 May 2015 19:21
PMCID: PMC394824
URI: http://repository.cshl.edu/id/eprint/31483

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