The myb-homologous P gene controls phlobaphene pigmentation in maize floral organs by directly activating a flavonoid biosynthetic gene subset

Grotewold, E., Drummond, B. J., Bowen, B., Peterson, T. (February 1994) The myb-homologous P gene controls phlobaphene pigmentation in maize floral organs by directly activating a flavonoid biosynthetic gene subset. Cell, 76 (3). pp. 543-53. ISSN 0092-8674 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/8313474
DOI: 10.1016/0092-8674(94)90117-1

Abstract

The maize P gene, which specifies red pigmentation of the kernel pericarp, cob, and other floral organs, has been an important model since the early days of modern genetics. Here we show that P encodes a Myb homolog that recognizes the sequence CCT/AACC, in sharp contrast with the C/TAACGG bound by vertebrate Myb proteins. P binds to and activates transcription of the A1 gene required for 3-deoxy flavonoid and phlobaphene biosynthesis, but not the Bz1 gene required for anthocyanin biosynthesis. The maize C1 gene, which also encodes a Myb homolog, activates both the A1 and Bz1 genes, but only in the presence of a basic-helix-loop-helix coactivator encoded by the maize genes R or B. These results indicate that Myb homologs can differentially regulate gene expression by binding different DNA sequences, through combinatorial interactions with other factors, or both.

Item Type: Paper
Uncontrolled Keywords: Animals Base Sequence Binding Sites Comparative Study DNA-Binding Proteins/ genetics/metabolism Flavonoids/ biosynthesis Gene Expression Regulation Genes, Plant Genes, Regulator Molecular Sequence Data Mutagenesis Oncogene Proteins v-myb Oncogenes Pigments, Biological/biosynthesis Plant Proteins/ genetics/metabolism Promoter Regions (Genetics) Research Support, U.S. Gov't, Non-P.H.S. Retroviridae Proteins, Oncogenic/biosynthesis/genetics Sequence Homology, Nucleic Acid Transcription, Genetic Vertebrates Zea mays/ genetics/physiology
Subjects: organism description > plant > maize
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
CSHL Authors:
Communities: CSHL labs > Grotewold lab
Depositing User: Matt Covey
Date: 11 February 1994
Date Deposited: 04 May 2015 19:09
Last Modified: 04 May 2015 19:09
Related URLs:
URI: http://repository.cshl.edu/id/eprint/31482

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